Journal: Advanced Healthcare Materials

Article Title: An Engineered Biomimetic Nanoreactor With Cascade Functions for Targeted and Combinatorial Therapy of Ischemic Stroke

doi: 10.1002/adhm.71712

Figure Lengend Snippet: Preparation and characterization of RM@HPAN. TEM images of (A) HMPB; (B) HPAN; (C) M@HPAN; and (D) RM@HPAN. (E) Elemental mapping of RM@HPAN. (F) Particle size distribution of HMPB, HPAN, M@HPAN, RM@HPAN. (G) Zeta potentials of HMPB, HPAN, M@HPAN, RM@HPAN. (H) UV−vis spectroscopy of PB, HMPB, Arg, NBP, and HPAN. (I) FTIR spectroscopy of HMPB, and HPAN. (J) The isotherm adsorption–desorption curve of HMPB. (K) XRD patterns of HMPB. (L) Variation in mean particle sizes of RM@HPAN in PBS solution. (M) Western blot analysis of CD47 and Band 3 protein of different groups. Data were expressed as mean ± SD ( n = 3).

Article Snippet: Membranes were blocked with 5% non‐fat milk in TBST (Tris‐buffered saline with 0.1% Tween‐20) for 1 h at room temperature, then incubated overnight at 4°C with primary antibodies against the following targets: CD47 (Proteintech, 1:1000), Band 3 (Proteintech, 1:500), CHRNA7 (Abcam, 1:1000), Rap1 (Affinity, 1:1000), Rap1‐GTP (Affinity, 1:500), PI3K (Affinity, 1:1000), p‐PI3K (Invitrogen, 1:1000), Akt (Abcam, 1:1000), p‐Akt (Bioss, 1:1000), Bcl‐2 (Bioss, 1:1000), Bax (Proteintech, 1:1000), cleaved caspase‐3 (CST, 1:1000), p‐NF‐κB p65 (Affinity, 1:1000), Nrf2 (ZEN BIO, 1:1000), HO‐1 (ZEN BIO, 1:1000), BVR‐B (Huamei, 1:1000), β‐actin (Affinity, 1:2000), GAPDH (Proteintech, 1:2000), and Lamin B (Boster Bioengineering, 1:1000).

Techniques: UV-Vis Spectroscopy, Fourier Transform Infrared Spectroscopy, Adsorption, Western Blot, Analysis, Zeta Potential Analyzer, Pore Size